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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Reviews (5)Publications (62) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Mouse

ABclonal:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:500 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)

Overview

Product namePhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
Catalog No.AP0472
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
ImmunogenA synthetic phosphorylated peptide around T185 & Y187 of human ERK2MAPK1 (NP_002736.3).
SequenceLTEYV
Gene ID55945595
Swiss protP28482P27361
SynonymsMAPK1/MAPK3
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42KDa/44KDa
ReactivityMouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesNIH/3T3
Cellular location
Customer validation

(Mouse embryonal fibroblast cell, MDA-MB-231, Raw264.7, C6, Mouse splenocytes)

WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus, Mycobacterium tuberculosis, Capra hircus)

IF(Homo sapiens)

IP(Homo sapiens)

    ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)}

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:500 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

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