Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Reviews (5)Publications (42) Datasheet

ABclonal:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Immunohistochemistry of paraffin-embedded human colon using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472). Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)
Review
ABclonal: review for Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb(AP0472)

Overview

Product namePhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
Catalog No.AP0472
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
ImmunogenA synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
SequenceFLTEYVA
Gene ID5594/5595
Swiss protP28482/P27361
SynonymsMAPK1/MAPK3
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42KDa/44KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesJurkat, NIH/3T3, C6
Cellular location
Customer validation

(Mouse embryonal fibroblast cell, MDA-MB-231, Raw264.7, C6, Mouse splenocytes)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Sus scrofa, Mycobacterium tuberculosis)

IP(Homo sapiens)

IF(Homo sapiens)

    ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) }

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) }

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Immunohistochemistry of paraffin-embedded human colon using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) }

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) }

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
    ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) }

    Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472)

    Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472). Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

    * For research use only. Not for therapeutic or diagnostic purposes.

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