Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

Publications (3) Datasheet

ABclonal:Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)
ABclonal:Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

Overview

Product namePhospho-ERK1-T202 + ERK2-T185 Rabbit mAb
Catalog No.AP0485
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185).
SequenceEmail for sequence
Gene ID5595/5594
Swiss protP27361/P28482
SynonymsH3.4; H3/g; H3FT; H3t; H3/A; H3FA; ERK1 / ERK2
Calculated MW42, 44kDa
Observed MW44KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, NIH/3T3
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

    ABclonal:Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) }

    Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

    Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) }

    Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

    Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1S.
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) }

    Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

    Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) }

    Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

    Immunohistochemistry of paraffin-embedded human appendix using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) }

    Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485)

    Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).

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