Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-EIF4B-S422 Rabbit pAb (AP0278)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-EIF4B-S422 Rabbit pAb (AP0278)

Western blot analysis of extracts from HeLa cells using phospho-EIF4B-S422 antibody (AP0278).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-EIF4B-S422 Rabbit pAb
Catalog No. AP0278
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S422 of human EIF4B
Sequence Email for sequence
Gene ID 1975
Swiss prot P23588
Synonyms EIF-4B; PRO1843; Phospho-EIF4B-S422
Calculated MW 69kDa
Observed MW 75kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location cytosol, eukaryotic translation initiation factor 4F complex

Research Area

Phospho-EIF4B-S422 Rabbit pAb images

ABclonal:Western blot - Phospho-EIF4B-S422 Rabbit pAb (AP0278)}

Western blot - Phospho-EIF4B-S422 Rabbit pAb (AP0278)

Western blot analysis of extracts from HeLa cells using phospho-EIF4B-S422 antibody (AP0278).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF4B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF4B. (Distance between topics and target gene indicate popularity.) EIF4B

* Data provided by citexs.com, for reference only.

Publishing research using AP0278? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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