Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PKR/EIF2AK2-T446 Rabbit mAb (AP0632)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-PKR/EIF2AK2-T446 Rabbit mAb (AP0632)

Western blot analysis of extracts of HeLa cells, using Phospho-PKR/EIF2AK2-T446 antibody (AP0632).HeLa cells were treated by TNF-α (20 ng/mL) and Calyculin A (100 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-PKR/EIF2AK2-T446 Rabbit mAb
Catalog No. AP0632
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T446 of human PKR/EIF2AK2
Sequence Email for sequence
Gene ID 5610
Swiss prot P19525
Synonyms PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; Phospho-PKR/EIF2AK2-T446
Calculated MW 62kDa
Observed MW 62kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus, perinuclear region
Customer validation

WB (Rattus norvegicus)

Research Area

Phospho-PKR/EIF2AK2-T446 Rabbit mAb images

ABclonal:Western blot - Phospho-PKR/EIF2AK2-T446 Rabbit mAb (AP0632)}

Western blot - Phospho-PKR/EIF2AK2-T446 Rabbit mAb (AP0632)

Western blot analysis of extracts of HeLa cells, using Phospho-PKR/EIF2AK2-T446 antibody (AP0632).HeLa cells were treated by TNF-α (20 ng/mL) and Calyculin A (100 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF2AK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF2AK2. (Distance between topics and target gene indicate popularity.) EIF2AK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0632? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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