Publications (2) Datasheet SDS COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human
Western blot analysis of extracts of BxPC-3 cells, using Phospho-EGFR-Y1173 Rabbit mAb (AP0992) at 1:1000 dilution.BxPC-3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Product name | Phospho-EGFR-Y1173 Rabbit mAb |
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Catalog No. | AP0992 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
CloneNo. | ARC1536 |
Immunogen | A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR |
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Sequence | Email for sequence |
Gene ID | 1956 |
Swiss prot | P00533 |
Synonyms | ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; Phospho-EGFR-Y1173 |
Calculated MW | 134kDa |
Observed MW | 175kDa |
Reactivity | Human |
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Tested applications | Testing results |
WB | Human |
Recommended dilution |
|
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Application key | Western blotting |
Positive samples | BxPC-3+EGF |
Cellular location | Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein |
Customer validation | WB (Homo sapiens) IP (Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on EGFR. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to EGFR. (Distance between topics and target gene indicate popularity.) EGFR
* Data provided by citexs.com, for reference only.
Publishing research using AP0992? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.