Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Western blot analysis of extracts of HeLa cells, using Phospho-EGFR-Y1068 pAb (AP0301) at 1:1000 dilution or EGFR antibody (A11351).HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-Y1068 pAb (AP0301). Western blot was performed from the immunoprecipitate using Phospho-EGFR-Y1068 pAb (AP0301) at a dilition of 1:1000. A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

Review
ABclonal: review for Phospho-EGFR-Y1068 Rabbit pAb(AP0301)
Review
ABclonal: review for Phospho-EGFR-Y1068 Rabbit pAb(AP0301)

Overview

Product namePhospho-EGFR-Y1068 Rabbit pAb
Catalog No.AP0301
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y1068 of human EGFR
SequenceEmail for sequence
Gene ID1956
Swiss protP00533
SynonymsEGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
Calculated MW44kDa/69kDa/77kDa/134kDa
Observed MW185kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

(Human non small cell lung adenocarcinoma cell)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ABclonal:Western blot - Phospho-EGFR-Y1068 Rabbit pAb (AP0301) }

Western blot - Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Western blot analysis of extracts of HeLa cells, using Phospho-EGFR-Y1068 pAb (AP0301) at 1:1000 dilution or EGFR antibody (A11351).HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-EGFR-Y1068 Rabbit pAb (AP0301) }

Immunoprecipitation - Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-Y1068 pAb (AP0301). Western blot was performed from the immunoprecipitate using Phospho-EGFR-Y1068 pAb (AP0301) at a dilition of 1:1000. A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

Submit an review for AP0301

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about AP0301 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AP0301? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order