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Phospho-EGFR-T669 Rabbit pAb (AP0025)

Review (1) Datasheet

ABclonal:Western blot - Phospho-EGFR-T669 Rabbit pAb (AP0025)

Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody (AP0025) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - Phospho-EGFR-T669 Rabbit pAb (AP0025)

Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-T669 pAb (AP0025). Western blot was performed from the immunoprecipitate using Phospho-EGFR-T669 pAb (AP0025) at a dilition of 1:1000. A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

Review
ABclonal: review for Phospho-EGFR-T669 Rabbit pAb(AP0025)

Overview

Product namePhospho-EGFR-T669 Rabbit pAb
Catalog No.AP0025
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
ImmunogenA phospho specific peptide corresponding to residues surrounding T669 of human EGFR
SequenceEmail for sequence
Gene ID1956
Swiss protP00533
SynonymsEGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
Calculated MW44kDa/69kDa/77kDa/134kDa
Observed MW200kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesA-431, HUVEC
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

(A551)

ABclonal:Western blot - Phospho-EGFR-T669 Rabbit pAb (AP0025) }

Western blot - Phospho-EGFR-T669 Rabbit pAb (AP0025)

Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody (AP0025) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - Phospho-EGFR-T669 Rabbit pAb (AP0025) }

Immunoprecipitation - Phospho-EGFR-T669 Rabbit pAb (AP0025)

Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-T669 pAb (AP0025). Western blot was performed from the immunoprecipitate using Phospho-EGFR-T669 pAb (AP0025) at a dilition of 1:1000. A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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