Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-CRMP2/DPYSL2-T514 Rabbit pAb
Catalog No. AP0821
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T514 of human CRMP2/DPYSL2 (NP_001377.1).
Sequence TVTPA
Gene ID 1808
Swiss prot Q16555
Synonyms DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2; Phospho-CRMP2/DPYSL2-T514
Calculated MW 62kDa
Observed MW 69kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples SH-SY5Y, PC-12
Cellular location Cytoplasm, Membrane, cytoskeleton, cytosol

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-CRMP2/DPYSL2-T514 Rabbit pAb images

ABclonal:Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)}

Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on DPYSL2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DPYSL2. (Distance between topics and target gene indicate popularity.) DPYSL2

* Data provided by citexs.com, for reference only.

Publishing research using AP0821? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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