Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263)

Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Phospho-Cytokeratin 18 (KRT18)-S33 antibody (AP0263).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb
Catalog No. AP0263
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S33 of human KRT18
Sequence Email for sequence
Gene ID 3875
Swiss prot P05783
Synonyms K18; CK-18; CYK18; Phospho-Cytokeratin 18 (KRT18)-S33
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HT-29
Cellular location Cytoplasm, Nucleus, nucleolus, perinuclear region
Customer validation

WB (Homo sapiens)

Research Area

Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb images

ABclonal:Western blot - Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263)}

Western blot - Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263)

Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Phospho-Cytokeratin 18 (KRT18)-S33 antibody (AP0263).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on KRT18. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KRT18. (Distance between topics and target gene indicate popularity.) KRT18

* Data provided by citexs.com, for reference only.

Publishing research using AP0263? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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