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Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Publications (3) Datasheet

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (AP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of C2C12 cells, using Phospho-β-Catenin-S552 pAb (AP0579) at 1:1000 dilution or β-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product namePhospho-β-Catenin-S552 Rabbit pAb
Catalog No.AP0579
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic phosphorylated peptide around S552 of human beta Catenin (NP_001091679).
SequenceRTSMG
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW9kDa/85kDa
Observed MW106kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesC2C12, NIH/3T3
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

WB(Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579) }

Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (AP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579) }

Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of C2C12 cells, using Phospho-β-Catenin-S552 pAb (AP0579) at 1:1000 dilution or β-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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