Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Cortactin-Y466 Rabbit pAb (AP0247)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-Cortactin-Y466 Rabbit pAb (AP0247)

Western blot analysis of extracts from Jurkat cells using Phospho-Cortactin-Y466 antibody (AP0247).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Cortactin-Y466 Rabbit pAb
Catalog No. AP0247
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y466 of human Cortactin
Sequence Email for sequence
Gene ID 2017
Swiss prot Q14247
Synonyms EMS1; Phospho-Cortactin-Y466
Calculated MW 62kDa
Observed MW 80kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton, dendrite, dendritic spine, focal adhesion, lamellipodium, podosome, ruffle

Research Area

Phospho-Cortactin-Y466 Rabbit pAb images

ABclonal:Western blot - Phospho-Cortactin-Y466 Rabbit pAb (AP0247)}

Western blot - Phospho-Cortactin-Y466 Rabbit pAb (AP0247)

Western blot analysis of extracts from Jurkat cells using Phospho-Cortactin-Y466 antibody (AP0247).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on CTTN. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CTTN. (Distance between topics and target gene indicate popularity.) CTTN

* Data provided by citexs.com, for reference only.

Publishing research using AP0247? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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