Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Immunohistochemistry analysis of Phospho-CRKL-Y207 in paraffin-embedded rat brain using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name Phospho-CRKL-Y207 Rabbit pAb
Catalog No. AP0824
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
Sequence HAYAQ
Gene ID 1399
Swiss prot P46109
Synonyms CRKL; Phospho-CRKL-Y207
Calculated MW 34kDa
Observed MW 38kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples K-562
Cellular location cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-CRKL-Y207 Rabbit pAb images

ABclonal:Western blot - Phospho-CRKL-Y207 Rabbit pAb (AP0824)}

Western blot - Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Phospho-CRKL-Y207 Rabbit pAb (AP0824)}

Immunohistochemistry - Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Immunohistochemistry analysis of Phospho-CRKL-Y207 in paraffin-embedded rat brain using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about AP0824 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on CRKL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CRKL. (Distance between topics and target gene indicate popularity.) CRKL

* Data provided by citexs.com, for reference only.

Publishing research using AP0824? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order