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Phospho-CREB1-S133 pAb (AP0019)

Publication (2) Datasheet

ABclonal:Western blot - Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019)

Overview

Product namePhospho-CREB1-S133 pAb
Catalog No.AP0019
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
ImmunogenA synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).
SequenceRPSYR
Gene ID1385
Swiss protP16220
SynonymsCREB1; CREB; CREB-1; cAMP responsive element binding protein 1
Calculated MW25kDa/35kDa/36kDa
Observed MW43kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa
Cellular locationNucleus
Customer validation

WB(Human, Rat)

ABclonal:Western blot - Phospho-CREB1-S133 pAb (AP0019) }

Western blot - Phospho-CREB1-S133 pAb (AP0019)

Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 antibody (AP0019) at 1:1000 dilution or CREB1 antibody (A11989). HeLa cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019) }

Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019)

Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody (AP0019). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019) }

Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-CREB1-S133 pAb (AP0019). Western blot was performed from the immunoprecipitate using Phospho-CREB1-S133 pAb (AP0019) at a dilition of 1:1000. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.

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