Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 pAb (AP0019) at 1:2000 dilution or CREB1 antibody (A11064).HeLa cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human appendix using Phospho-CREB1-S133 antibody (AP0019) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal using Phospho-CREB1-S133 antibody (AP0019) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody (AP0019). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-CREB1-S133 pAb (AP0019). Western blot was performed from the immunoprecipitate using Phospho-CREB1-S133 pAb (AP0019) at a dilition of 1:1000. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
|Product name||Phospho-CREB1-S133 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:200 - 1:2000|
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation|
WB(Homo sapiens, Danio rerio, Rattus norvegicus, Mus musculus)
* For research use only. Not for therapeutic or diagnostic purposes.
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