Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-CFL1-Y139 Rabbit pAb
Catalog No. AP0330
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1).
Sequence ANCYE
Gene ID 1072
Swiss prot P23528
Synonyms CFL; cofilin; HEL-S-15; Phospho-CFL1-Y139
Calculated MW 19kDa
Observed MW Refer to figures

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Immunohistochemistry    
Positive samples
Cellular location Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane

Documents

Certificate of Compliance

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Research Area

Phospho-CFL1-Y139 Rabbit pAb images

ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)}

Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)}

Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)}

Immunohistochemistry - Phospho-CFL1-Y139 Rabbit pAb (AP0330)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CFL1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CFL1. (Distance between topics and target gene indicate popularity.) CFL1

* Data provided by citexs.com, for reference only.

Publishing research using AP0330? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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