Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDK9-T186 Rabbit pAb (AP0810)

Reviews (2) Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CDK9-T186 Rabbit pAb (AP0810)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (AP0810) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for Phospho-CDK9-T186 Rabbit pAb(AP0810)
Review
ABclonal: review for Phospho-CDK9-T186 Rabbit pAb(AP0810)

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Overview

Product name Phospho-CDK9-T186 Rabbit pAb
Catalog No. AP0810
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T186 of human CDK9 (NP_001252.1).
Sequence RYTNR
Gene ID 1025
Swiss prot P50750
Synonyms TAK; C-2k; CTK1; CDC2L4; PITALRE; Phospho-CDK9-T186
Calculated MW 43kDa
Observed MW 43kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3
Cellular location Cytoplasm, Nucleus, PML body
Customer validation

WB (Human fibrosarcoma cells, HeLa cellsMus musculus, Anatinae)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-CDK9-T186 Rabbit pAb images

ABclonal:Western blot - Phospho-CDK9-T186 Rabbit pAb (AP0810)}

Western blot - Phospho-CDK9-T186 Rabbit pAb (AP0810)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (AP0810) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK9. (Distance between topics and target gene indicate popularity.) CDK9

* Data provided by citexs.com, for reference only.

Publishing research using AP0810? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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