Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDK6-Y24 Rabbit pAb (AP0289)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-CDK6-Y24 Rabbit pAb (AP0289)

Western blot analysis of extracts from 293 cells using Phospho-CDK6-Y24 antibody (AP0289).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-CDK6-Y24 Rabbit pAb
Catalog No. AP0289
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y24 of human CDK6
Sequence Email for sequence
Gene ID 1021
Swiss prot Q00534
Synonyms MCPH12; PLSTIRE; Phospho-CDK6-Y24
Calculated MW 37kDa
Observed MW 37kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293
Cellular location Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle

Research Area

Phospho-CDK6-Y24 Rabbit pAb images

ABclonal:Western blot - Phospho-CDK6-Y24 Rabbit pAb (AP0289)}

Western blot - Phospho-CDK6-Y24 Rabbit pAb (AP0289)

Western blot analysis of extracts from 293 cells using Phospho-CDK6-Y24 antibody (AP0289).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK6. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK6. (Distance between topics and target gene indicate popularity.) CDK6

* Data provided by citexs.com, for reference only.

Publishing research using AP0289? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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