Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDK2-T160 Rabbit pAb (AP0325)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit pAb (AP0325) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Western blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit pAb (AP0325) at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37℃ for 20 hours.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 3s.

ABclonal:Immunohistochemistry - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Immunohistochemistry analysis of Phospho-CDK2-T160 in paraffin-embedded human breast carcinoma using Phospho-CDK2-T160 Rabbit pAb (AP0325).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-CDK2-T160 Rabbit pAb
Catalog No. AP0325
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T160 of human CDK2 (NP_001789.2?/NP_001249.1).
Sequence TYTHE
Gene ID 1017
Swiss prot P24941
Synonyms CDKN2; p33(CDK2); Phospho-CDK2-T160
Calculated MW 34kDa
Observed MW 32kDa/34kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6,
Cellular location Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB (Tilapia, Homo sapiens)

IF (Tilapia)

IHC (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-CDK2-T160 Rabbit pAb images

ABclonal:Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)}

Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit pAb (AP0325) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)}

Western blot - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Western blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit pAb (AP0325) at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37℃ for 20 hours.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 3s.
ABclonal:Immunohistochemistry - Phospho-CDK2-T160 Rabbit pAb (AP0325)}

Immunohistochemistry - Phospho-CDK2-T160 Rabbit pAb (AP0325)

Immunohistochemistry analysis of Phospho-CDK2-T160 in paraffin-embedded human breast carcinoma using Phospho-CDK2-T160 Rabbit pAb (AP0325).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK2. (Distance between topics and target gene indicate popularity.) CDK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0325? Please let us know so that we can cite the reference in this datasheet.

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* For research use only. Not for therapeutic or diagnostic purposes.

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