Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Reviews (2) Publications (17) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK1-Y15 Rabbit pAb (A0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of various lysates using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of lysates from C6 cells, using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution.C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded rat liver using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded human colon using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded mouse brain using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunofluorescence analysis of paraffin-embedded human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilution of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.

Review
ABclonal: review for Phospho-CDK1-Y15 Rabbit pAb(AP0016)
Review
ABclonal: review for Phospho-CDK1-Y15 Rabbit pAb(AP0016)

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Overview

Product name Phospho-CDK1-Y15 Rabbit pAb
Catalog No. AP0016
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).
Sequence GTYGV
Gene ID 983
Swiss prot P06493
Synonyms CDC2; CDC28A; P34CDC2; Phospho-CDK1-Y15
Calculated MW 34kDa
Observed MW 34kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HT-29, HeLa, NIH/3T3, C6
Cellular location Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

WB (H460,A549, HeLaHomo sapiens, Mus musculus, Homo sapiens, Eriocheir sinensis)

IF (Homo sapiens, Mus musculus)

IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-CDK1-Y15 Rabbit pAb images

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK1-Y15 Rabbit pAb (A0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of various lysates using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of lysates from C6 cells, using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution.C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded rat liver using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded human colon using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry analysis of Phospho-CDK1-Y15 in paraffin-embedded mouse brain using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunofluorescence analysis of paraffin-embedded human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016)}

Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilution of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK1. (Distance between topics and target gene indicate popularity.) CDK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0016? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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