Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Reviews (2)Publications (9) Datasheet

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-Y15 antibody (AP0016) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0016) at 1:1000 dilution or CDK1 antibody (A0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded human colon using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunofluorescence analysis of human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilition of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.

Review
ABclonal: review for Phospho-CDK1-Y15 Rabbit pAb(AP0016)
Review
ABclonal: review for Phospho-CDK1-Y15 Rabbit pAb(AP0016)

Overview

Product namePhospho-CDK1-Y15 Rabbit pAb
Catalog No.AP0016
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).
SequenceGTYGV
Gene ID983
Swiss protP06493
SynonymsCDK1; CDC2; CDC28A; P34CDC2
Calculated MW27kDa/34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHT-29, HeLa
Cellular locationCytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

(H460,A549, HeLa)

WB(Homo sapiens, Mus musculus, Eriocheir sinensis)

IF(Homo sapiens, Mus musculus)

ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-Y15 antibody (AP0016) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Western blot - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0016) at 1:1000 dilution or CDK1 antibody (A0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded human colon using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Immunohistochemistry - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Immunofluorescence - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunofluorescence analysis of human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016) }

Immunoprecipitation - Phospho-CDK1-Y15 Rabbit pAb (AP0016)

Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilition of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.

* For research use only. Not for therapeutic or diagnostic purposes.

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