Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDK1-T161 Rabbit pAb (AP0324)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (AP0324) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Rat liver tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Human spleen tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Human liver cancer tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Mouse liver tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunofluorescence analysis of Human tonsil tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunofluorescence analysis of Rat testis tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

ABclonal:Immunoprecipitation - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-CDK1-T161 pAb (AP0324). Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb (AP0324) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Overview

Product name Phospho-CDK1-T161 Rabbit pAb
Catalog No. AP0324
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T161 of human CDK1 (NP_001777.1).
Sequence VYTHE
Gene ID 983
Swiss prot P06493
Synonyms CDC2; CDC28A; P34CDC2; Phospho-CDK1-T161
Calculated MW 34kDa
Observed MW 34kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples HeLa
Cellular location Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

WB (Sus scrofa, Homo sapiens)

IP (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-CDK1-T161 Rabbit pAb images

ABclonal:Western blot - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Western blot - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (AP0324) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Rat liver tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Human spleen tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Human liver cancer tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunohistochemistry - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunohistochemistry analysis of Phospho-CDK1-T161 in paraffin-embedded Mouse liver tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunofluorescence analysis of Human tonsil tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunofluorescence - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunofluorescence analysis of Rat testis tissue using Phospho-CDK1-T161 Rabbit pAb (AP0324) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
ABclonal:Immunoprecipitation - Phospho-CDK1-T161 Rabbit pAb (AP0324)}

Immunoprecipitation - Phospho-CDK1-T161 Rabbit pAb (AP0324)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-CDK1-T161 pAb (AP0324). Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb (AP0324) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK1. (Distance between topics and target gene indicate popularity.) CDK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0324? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

Secondary Antibodies (23)

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