Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CDC25C-S216 Rabbit pAb (AP0323)

Review (1) Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-CDC25C-S216 Rabbit pAb (AP0323)

Western blot analysis of extracts from 293 cells using Phospho-CDC25C-S216 antibody (AP0323).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Review
ABclonal: review for Phospho-CDC25C-S216 Rabbit pAb(AP0323)

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Overview

Product name Phospho-CDC25C-S216 Rabbit pAb
Catalog No. AP0323
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S216 of human CDC25C
Sequence Email for sequence
Gene ID 995
Swiss prot P30307
Synonyms CDC25; PPP1R60; Phospho-CDC25C-S216
Calculated MW 53kDa
Observed MW 80kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293
Cellular location Nucleus
Customer validation

WB (Human lung cancer cellHomo sapiens)

Research Area

Phospho-CDC25C-S216 Rabbit pAb images

ABclonal:Western blot - Phospho-CDC25C-S216 Rabbit pAb (AP0323)}

Western blot - Phospho-CDC25C-S216 Rabbit pAb (AP0323)

Western blot analysis of extracts from 293 cells using Phospho-CDC25C-S216 antibody (AP0323).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDC25C. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDC25C. (Distance between topics and target gene indicate popularity.) CDC25C

* Data provided by citexs.com, for reference only.

Publishing research using AP0323? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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