Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-CBL-Y774 Rabbit pAb (AP0794)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)

Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (A0732).Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.

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Overview

Product name Phospho-CBL-Y774 Rabbit pAb
Catalog No. AP0794
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y774 of human CBL (NP_005179.2).
Sequence DGYDV
Gene ID 867
Swiss prot P22681
Synonyms CBL2; NSLL; C-CBL; RNF55; FRA11B; Phospho-CBL-Y774
Calculated MW 100kDa
Observed MW 110kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Cell membrane, Cytoplasm

Research Area

Phospho-CBL-Y774 Rabbit pAb images

ABclonal:Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)}

Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)

Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (A0732).Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.

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Related Targets

Based on existing publications, the following genes are closely related to research on CBL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CBL. (Distance between topics and target gene indicate popularity.) CBL

* Data provided by citexs.com, for reference only.

Publishing research using AP0794? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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