Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252)

Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name Phospho-Beclin 1-Ser15 Rabbit pAb
Catalog No. AP1252
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1).
Sequence QVSFV
Gene ID 8678
Swiss prot Q14457
Synonyms ATG6; VPS30; beclin1; Phospho-Beclin 1-Ser15
Calculated MW 52kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3, C6
Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Beclin 1-Ser15 Rabbit pAb images

ABclonal:Western blot - Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252)}

Western blot - Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252)

Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on BECN1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BECN1. (Distance between topics and target gene indicate popularity.) BECN1

* Data provided by citexs.com, for reference only.

Publishing research using AP1252? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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