Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (AP0232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5μg/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-BRCA1-S1423 Rabbit pAb
Catalog No. AP0232
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
Sequence HGSQP
Gene ID 672
Swiss prot P38398
Synonyms IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53; Phospho-BRCA1-S1423
Calculated MW 208kDa
Observed MW 270kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HT-29
Cellular location Chromosome, Cytoplasm, Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-BRCA1-S1423 Rabbit pAb images

ABclonal:Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (AP0232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5μg/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BRCA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BRCA1. (Distance between topics and target gene indicate popularity.) BRCA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0232? Please let us know so that we can cite the reference in this datasheet.

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* For research use only. Not for therapeutic or diagnostic purposes.

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