Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (AP0232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-BRCA1-S1423 Rabbit pAb
Catalog No.AP0232
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Immunogen information

ImmunogenA synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
SequenceHGSQP
Gene ID672
Swiss protP38398
SynonymsBRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Calculated MW7kDa/78-85kDa/202-210kDa
Observed MW270kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHT-29
Cellular locationChromosome, Cytoplasm, Cytoplasm, Nucleus

Research Area

Phospho-BRCA1-S1423 Rabbit pAb images

ABclonal:Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Western blot - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (AP0232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunohistochemistry - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)}

Immunofluorescence - Phospho-BRCA1-S1423 Rabbit pAb (AP0232)

Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (AP0232) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BRCA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BRCA1. (Distance between topics and target gene indicate popularity.) BRCA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0232? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (6)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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