Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Bcl-2-S70 Rabbit pAb (AP0575)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)

Western blot analysis of lysates from Jurkat and 293 cells, using Phospho-Bcl-2-S70 Rabbit pAb (AP0575) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunofluorescence - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)

Immunofluorescence analysis of HeLa cells using Phospho-Bcl-2-S70 Rabbit pAb (AP0575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-Bcl-2-S70 Rabbit pAb
Catalog No. AP0575
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S70 of human Bcl-2 (NP_000624.2).
Sequence RTSPL
Gene ID 596
Swiss prot P10415
Synonyms Bcl-2; PPP1R50; Phospho-Bcl-2-S70
Calculated MW 26kDa
Observed MW 28kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Jurkat, 293
Cellular location Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Bcl-2-S70 Rabbit pAb images

ABclonal:Western blot - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)}

Western blot - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)

Western blot analysis of lysates from Jurkat and 293 cells, using Phospho-Bcl-2-S70 Rabbit pAb (AP0575) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunofluorescence - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)}

Immunofluorescence - Phospho-Bcl-2-S70 Rabbit pAb (AP0575)

Immunofluorescence analysis of HeLa cells using Phospho-Bcl-2-S70 Rabbit pAb (AP0575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BCL2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BCL2. (Distance between topics and target gene indicate popularity.) BCL2

* Data provided by citexs.com, for reference only.

Publishing research using AP0575? Please let us know so that we can cite the reference in this datasheet.

Antibodies (12)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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