Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (AP0789) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name Phospho-BCAR1-Y410 Rabbit pAb
Catalog No. AP0789
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y410 of human BCAR1 (NP_055382.2).
Sequence GVYAV
Gene ID 9564
Swiss prot P56945
Synonyms CAS; CAS1; CASS1; CRKAS; P130Cas; Phospho-BCAR1-Y410
Calculated MW 93kDa
Observed MW 140kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Cell junction, Cytoplasm, focal adhesion

Research Area

Phospho-BCAR1-Y410 Rabbit pAb images

ABclonal:Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)}

Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (AP0789) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on BCAR1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BCAR1. (Distance between topics and target gene indicate popularity.) BCAR1

* Data provided by citexs.com, for reference only.

Publishing research using AP0789? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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