Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-ATM-S1981 Rabbit pAb (AP0008)

Datasheet

ABclonal:Western blot - Phospho-ATM-S1981 pAb (AP0008)
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)

Overview

Product namePhospho-ATM-S1981 Rabbit pAb
Catalog No.AP0008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
ImmunogenA synthetic phosphorylated peptide around S1981 of human ATM (NP_000042.3).
SequenceEGSQS
Gene ID472
Swiss protQ13315
SynonymsAT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1; ATM
Calculated MW350kDa
Observed MW350kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasmic vesicle, Nucleus
ABclonal:Western blot - Phospho-ATM-S1981 pAb (AP0008) }

Western blot - Phospho-ATM-S1981 pAb (AP0008)

Western blot analysis of extracts of 293T cells, using Phospho-ATM-S1981 antibody (AP0008) at 1:2000 dilution. 293T cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008) }

Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)

Immunofluorescence analysis of MCF-7 cells using Phospho-ATM-S1981 antibody (AP0008). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008) }

Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)

Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody (AP0008). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008) }

Immunofluorescence - Phospho-ATM-S1981 pAb (AP0008)

Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody (AP0008). Blue: DAPI for nuclear staining.

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