Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-ATF2-T69/T51 Rabbit pAb (AP0128)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ATF2-T69/T51 Rabbit pAb (AP0128)

Western blot analysis of extracts from 3T3 cells using Phospho-ATF2-T69/T51 antibody (AP0128).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-ATF2-T69/T51 Rabbit pAb
Catalog No. AP0128
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T69/T51 of human ATF2
Sequence Email for sequence
Gene ID 1386
Swiss prot P15336
Synonyms HB16; CREB2; TREB7; CREB-2; CRE-BP1; Phospho-ATF2-T69/T51
Calculated MW 55kDa
Observed MW 75kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 3T3
Cellular location Cytoplasm, Mitochondrion outer membrane, Nucleus

Research Area

Phospho-ATF2-T69/T51 Rabbit pAb images

ABclonal:Western blot - Phospho-ATF2-T69/T51 Rabbit pAb (AP0128)}

Western blot - Phospho-ATF2-T69/T51 Rabbit pAb (AP0128)

Western blot analysis of extracts from 3T3 cells using Phospho-ATF2-T69/T51 antibody (AP0128).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on ATF2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ATF2. (Distance between topics and target gene indicate popularity.) ATF2

* Data provided by citexs.com, for reference only.

Publishing research using AP0128? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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