Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Reviews (4)Publications (13) Datasheet

ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (AP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.

Review
ABclonal: review for Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb(AP0116)

Overview

Product namePhospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb
Catalog No.AP0116
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
ImmunogenA phospho specific peptide corresponding to residues surrounding T172 of human AMPKα
SequenceEmail for sequence
Gene ID5562/5563
Swiss protQ13131/P54646
SynonymsAMPKa1/AMPKa2
Calculated MW64kDa/65kDa
Observed MW64KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesC2C12
Cellular location
Customer validation

(Human lung cancer cells, unknow, HeLa)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Danio rerio, Cavia porcellus)

IHC(Homo sapiens)

    ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116) }

    Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

    Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (AP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116) }

    Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

    Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116) }

    Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

    Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116) }

    Immunohistochemistry - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

    Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
    ABclonal:Immunoprecipitation - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116) }

    Immunoprecipitation - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0116)

    Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.

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