Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (AP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (AP0116) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.
|Product name||Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IHC 1:50 - 1:100
IP 1:50 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunoprecipitation|
(Human lung cancer cells, unknow, HeLa)
WB(Mus musculus, Homo sapiens, Rattus norvegicus, Danio rerio, Cavia porcellus)
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