Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171)

Publications (3) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171)

Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

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Overview

Product name Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb
Catalog No. AP1171
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).
Sequence LRTSC
Gene ID 55625563
Swiss prot Q13131P54646
Synonyms AMPKa1/AMPKa2; Phospho-AMPKa1/AMPKa2-T183/T172
Calculated MW 64kDa/65kDa/62kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, C2C12, C6
Cellular location apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Customer validation

WB (Rattus norvegicus, Mus musculus)

IHC (Rattus norvegicus)

IF (Rattus norvegicus)

ELISA (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb images

ABclonal:Western blot - Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171)}

Western blot - Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171)

Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKAA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAA1. (Distance between topics and target gene indicate popularity.) PRKAA1

* Data provided by citexs.com, for reference only.

Publishing research using AP1171? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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