Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-AKT2-S474 Rabbit pAb (AP0005)

Publications (3) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-AKT2-S474 Rabbit pAb (AP0005)

Western blot analysis of lysates from 293 cells, using Phospho-AKT2-S474 Rabbit pAb (AP0005) 1:1000 dilution..
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-AKT2-S474 Rabbit pAb
Catalog No. AP0005
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S474 of human AKT2 (NP_001617.1).
Sequence QFSYS
Gene ID 208
Swiss prot P31751
Synonyms PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2-S474
Calculated MW 56kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293
Cellular location Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein
Customer validation

WB (Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-AKT2-S474 Rabbit pAb images

ABclonal:Western blot - Phospho-AKT2-S474 Rabbit pAb (AP0005)}

Western blot - Phospho-AKT2-S474 Rabbit pAb (AP0005)

Western blot analysis of lysates from 293 cells, using Phospho-AKT2-S474 Rabbit pAb (AP0005) 1:1000 dilution..
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on AKT2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AKT2. (Distance between topics and target gene indicate popularity.) AKT2

* Data provided by citexs.com, for reference only.

Publishing research using AP0005? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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