Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Akt-Y315 Rabbit pAb (AP0274)

Publications (3) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded human brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded mouse brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded rat brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunofluorescence analysis of HeLa cells using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-Akt-Y315 Rabbit pAb
Catalog No. AP0274
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y315 of human AKT1 (NP_001014431.1).
Sequence PEYLA
Gene ID 20720810000
Swiss prot P31749P31751Q9Y243
Synonyms AKT1/AKT2/AKT3; Phospho-Akt-Y315
Calculated MW 48kDa/55kDa/51kDa/54kDa
Observed MW 56kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HepG2
Cellular location ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Akt-Y315 Rabbit pAb images

ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)}

Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded human brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)}

Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded mouse brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)}

Immunohistochemistry - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded rat brain using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-Akt-Y315 Rabbit pAb (AP0274)}

Immunofluorescence - Phospho-Akt-Y315 Rabbit pAb (AP0274)

Immunofluorescence analysis of HeLa cells using Phospho-Akt-Y315 Rabbit pAb (AP0274) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on AKT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AKT. (Distance between topics and target gene indicate popularity.) AKT

* Data provided by citexs.com, for reference only.

Publishing research using AP0274? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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