Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-AKT1-S473 pAb (AP0140)

Reviews (4)Publications (12) Datasheet

ABclonal:Western blot - Phospho-AKT1-S473 pAb (AP0140)
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 pAb(AP0140)

Overview

Product namePhospho-AKT1-S473 pAb
Catalog No.AP0140
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S473 of human AKT1
SequenceEmail for sequence
Gene ID207
Swiss protP31749
SynonymsAKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT serine/threonine kinase 1
Calculated MW48kDa/55kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

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WB(Mouse, Human)

IHC(Mouse)

ABclonal:Western blot - Phospho-AKT1-S473 pAb (AP0140) }

Western blot - Phospho-AKT1-S473 pAb (AP0140)

Western blot analysis of extracts from NIH/3T3 using Phospho-AKT1-S473 antibody (AP0140).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)

Immunohistochemistry of paraffin-embedded rat ovary using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)

Immunohistochemistry of paraffin-embedded human colon using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 pAb (AP0140)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).

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