Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-AKT1-S473 Rabbit pAb (AP0140)

Reviews (4)Publications (92) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Rat

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of Jurka cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.Jurkat cells were treated by Serum-starvation overnight at 37℃.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of C6 cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.C6 cells were treated by Serum-starvation overnight at 37℃.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)

Overview

Product namePhospho-AKT1-S473 Rabbit pAb
Catalog No.AP0140
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen information

ImmunogenA synthetic phosphorylated peptide around S473 of human Akt1 (NP_005154.2).
SequenceQFSYS
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW60KDa

Applications

ReactivityHuman, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesJurkat, C6
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(Human chronic myeloid leukemia cells, C57BL6, 293T, 3T3-L1)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Danio rerio, Dendrobium officinale Kimura et Migo, Bovine, Capra hircus, Apocynum venetum leaf, Mannan Oligosaccharide, Gallus gallus)

IHC(Mus musculus, Rattus norvegicus)

IF(Rattus norvegicus)

IHC(Homo sapiens,Mus musculus)

IF/ICC(Homo sapiens,Mus musculus)

IF(Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus)

WB(Rattus norvegicus)

Research Area

Phospho-AKT1-S473 Rabbit pAb images

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)}

Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of Jurka cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.Jurkat cells were treated by Serum-starvation overnight at 37℃.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)}

Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of C6 cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.C6 cells were treated by Serum-starvation overnight at 37℃.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on AKT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AKT1. (Distance between topics and target gene indicate popularity.) AKT1

* Data provided by citexs.com, for reference only.

Publishing research using AP0140? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (20)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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