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Phospho-AKT1-S473 Rabbit pAb (AP0140)

Reviews (4)Publications (44) Datasheet

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of Jurka cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.Jurkat cells were treated by Serum-starvation overnight at 37℃.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of C6 cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.C6 cells were treated by Serum-starvation overnight at 37℃.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded rat ovary using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded human colon using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).

Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit pAb(AP0140)

Overview

Product namePhospho-AKT1-S473 Rabbit pAb
Catalog No.AP0140
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S473 of human AKT1
SequenceEmail for sequence
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, C6
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(Human chronic myeloid leukemia cells, C57BL6, 293T, 3T3-L1)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Dendrobium officinale Kimura et Migo, Bovine, Capra hircus, Danio rerio)

IHC(Mus musculus)

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140) }

Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of Jurka cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.Jurkat cells were treated by Serum-starvation overnight at 37℃.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140) }

Western blot - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Western blot analysis of extracts of C6 cells, using Phospho-AKT1-S473 antibody (AP0140) at 1:1000 dilution.C6 cells were treated by Serum-starvation overnight at 37℃.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded rat ovary using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded human colon using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140) }

Immunohistochemistry - Phospho-AKT1-S473 Rabbit pAb (AP0140)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-AKT1-S473 antibody (AP0140) at dilution of 1:200 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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