Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473 pAb (AP0098) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-AKT1-S473 pAb (AP0098). Western blot was performed from the immunoprecipitate using Phospho-AKT1-S473 pAb (AP0098) at a dilition of 1:1000. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
|Product name||Phospho-AKT1-S473 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IP 1:50 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunoprecipitation|
|Cellular location||Cell membrane, Cytoplasm, Nucleus|
WB(Homo sapiens, Mus musculus, Rattus norvegicus)
IF(Homo sapiens, Rattus norvegicus)
* For research use only. Not for therapeutic or diagnostic purposes.
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