Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (A0282).C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded rat kidney using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded human appendix using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded mouse liver using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-c-Abl-Y412 Rabbit pAb
Catalog No. AP1060
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y412 of human ABL1 (NP_005148.2).
Sequence DTYTA
Gene ID 25
Swiss prot P00519
Synonyms ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; BCR-ABL; bcr/abl; Phospho-c-Abl-Y412
Calculated MW 123kDa
Observed MW 123kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6
Cellular location Cytoplasm, Lipid-anchor, Mitochondrion, Nucleus, Nucleus membrane, cytoskeleton

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-c-Abl-Y412 Rabbit pAb images

ABclonal:Western blot - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)}

Western blot - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (A0282).C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)}

Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded rat kidney using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)}

Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded human appendix using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)}

Immunohistochemistry - Phospho-c-Abl-Y412 Rabbit pAb (AP1060)

Immunohistochemistry analysis of Phospho-c-Abl-Y412 in paraffin-embedded mouse liver using Phospho-c-Abl-Y412 Rabbit pAb (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on ABL1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ABL1. (Distance between topics and target gene indicate popularity.) ABL1

* Data provided by citexs.com, for reference only.

Publishing research using AP1060? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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