Product Type > Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-Vimentin-S56 Antibody kit (RK06009)

Datasheet

ABclonal:Western blot - Phospho-Vimentin-S56 Rabbit pAb (AP0804)
ABclonal:Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)
ABclonal:Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)
ABclonal:Immunofluorescence - [KD Validated] Vimentin Rabbit pAb (A11952)

Overview

Product name Phospho-Vimentin-S56 Antibody kit
Catalog No. RK06009

Product Component

Product name Catalog No. Positive Applications Species Reactivity
Phospho-Vimentin-S56 Rabbit pAb AP0804 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Rat
[KD Validated] Vimentin Rabbit pAb A11952 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Mouse, Rat
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2).
A synthetic phosphorylated peptide around S56 of human VIM (NP_003371.2).
Sequence MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT
ASSPG
Gene ID 7431
Swiss prot P08670
Synonyms CTRCT30; HEL113; Vimentin; VIM; vimentin; Phospho-Vimentin-S56
Calculated MW 54kDa
Observed MW 57kDa
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Cellular location Cytoplasm
Phospho-Vimentin-S56 Rabbit pAb (AP0804)
ABclonal:Western blot - Phospho-Vimentin-S56 Rabbit pAb (AP0804)}
Western blot - Phospho-Vimentin-S56 Rabbit pAb (AP0804)

Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (AP0804) at 1:3000 dilution or Vimentin antibody (A11952).HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.


[KD Validated] Vimentin Rabbit pAb (A11952)
ABclonal:Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)}
Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)

Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)}
Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)

Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells, using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KD Validated] Vimentin Rabbit pAb (A11952)}
Immunofluorescence - [KD Validated] Vimentin Rabbit pAb (A11952)

Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb (A11952) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on VIM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VIM. (Distance between topics and target gene indicate popularity.) VIM

* Data provided by citexs.com, for reference only.

Publishing research using RK06009? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Discontinued

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