Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Rat
Product name | Phospho-Vimentin-S56 Antibody kit |
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Catalog No. | RK06009 |
Product name | Catalog No. | Positive Applications | Species Reactivity |
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Phospho-Vimentin-S56 Rabbit pAb | AP0804 | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition | Human, Rat |
[KD Validated] Vimentin Rabbit pAb | A11952 | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition | Human, Mouse, Rat |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2). A synthetic phosphorylated peptide around S56 of human VIM (NP_003371.2). |
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Sequence | MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT ASSPG |
Gene ID | 7431 |
Swiss prot | P08670 |
Synonyms | CTRCT30; HEL113; Vimentin; VIM; vimentin; Phospho-Vimentin-S56 |
Calculated MW | 54kDa |
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Observed MW | 57kDa |
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
Cellular location | Cytoplasm |
Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (AP0804) at 1:3000 dilution or Vimentin antibody (A11952).HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells, using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
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Based on existing publications, the following genes are closely related to research on VIM. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to VIM. (Distance between topics and target gene indicate popularity.) VIM
* Data provided by citexs.com, for reference only.
Publishing research using RK06009? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
Discontinued