Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-MAP2K1-S298 pAb (AP0063)

Publication (2) Datasheet

ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063)

Overview

Product namePhospho-MAP2K1-S298 pAb
Catalog No.AP0063
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
ImmunogenA phospho specific peptide corresponding to residues surrounding S298 of human MAP2K1
SequenceEmail for sequence
Gene ID5604
Swiss protQ02750
SynonymsCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Calculated MW40kDa/43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, C2C12, C6
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body
Customer validation

WB(Human)

ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063) }

Western blot - Phospho-MAP2K1-S298 pAb (AP0063)

Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (AP0063) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063) }

Western blot - Phospho-MAP2K1-S298 pAb (AP0063)

Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (AP0063) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063) }

Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063)

Immunoprecipitation analysis of 200ug extracts of 293 cells treated by PMA using 2.5ug Phospho-MAP2K1-S298 antibody (AP0063). Western blot was performed from the immunoprecipitate using Phospho-MAP2K1-S298 antibody (AP0063) at a dilition of 1:1000.

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