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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Reviews (4)Publications (15) Datasheet

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)


Product namePhospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
Catalog No.AP0472
Host speciesRabbit
Purification methodAffinity purification
ImmunogenA synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
Gene ID5594/5595
Swiss protP28482/P27361
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW38kDa, 41kDa
ReactivityHuman, Mouse, Rat
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesJurkat
Cellular location
Customer validation

WB(MDA-MB-231, Raw264.7, Mouse splenocytes, C6, Murine, Human, Mouse, Rat, Pig)

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }

Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:5000 dilution or ERK1/2 antibody (A16686).293T cells and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }

Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472). Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

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