Product Type > Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-CBL-Y774 Antibody kit (RK05788)

Datasheet

ABclonal:Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)
ABclonal:Western blot - CBL Rabbit pAb (A0732)
ABclonal:Western blot - CBL Rabbit pAb (A0732)
ABclonal:Immunohistochemistry - CBL Rabbit pAb (A0732)
ABclonal:Immunohistochemistry - CBL Rabbit pAb (A0732)
ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)
ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)
ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)
ABclonal:Immunoprecipitation - CBL Rabbit pAb (A0732)

Overview

Product name Phospho-CBL-Y774 Antibody kit
Catalog No. RK05788

Product Component

Product name Catalog No. Positive Applications Species Reactivity
Phospho-CBL-Y774 Rabbit pAb AP0794 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human
CBL Rabbit pAb A0732 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Mouse, Rat
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 850-906 of human CBL (NP_005179.2).
A synthetic phosphorylated peptide around Y774 of human CBL (NP_005179.2).
Sequence AATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT
DGYDV
Gene ID 867
Swiss prot P22681
Synonyms CBL2; NSLL; C-CBL; RNF55; FRA11B; Phospho-CBL-Y774
Calculated MW 100kDa
Observed MW 110kDa
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Cellular location Cell membrane,Cytoplasm
Phospho-CBL-Y774 Rabbit pAb (AP0794)
ABclonal:Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)}
Western blot - Phospho-CBL-Y774 Rabbit pAb (AP0794)

Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (A0732).Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.


CBL Rabbit pAb (A0732)
ABclonal:Western blot - CBL Rabbit pAb (A0732)}
Western blot - CBL Rabbit pAb (A0732)

Western blot analysis of lysates from 293T cells, using CBL Rabbit pAb (A0732) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - CBL Rabbit pAb (A0732)}
Western blot - CBL Rabbit pAb (A0732)

Western blot analysis of lysates from Mouse thymus, using CBL Rabbit pAb (A0732) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CBL Rabbit pAb (A0732)}
Immunohistochemistry - CBL Rabbit pAb (A0732)

Immunohistochemistry analysis of CBL in paraffin-embedded human prostate using CBL Rabbit pAb (A0732) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CBL Rabbit pAb (A0732)}
Immunohistochemistry - CBL Rabbit pAb (A0732)

Immunohistochemistry analysis of CBL in paraffin-embedded mouse lung using CBL Rabbit pAb (A0732) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)}
Immunofluorescence - CBL Rabbit pAb (A0732)

Immunofluorescence analysis of K-562 cells using CBL Rabbit pAb (A0732) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)}
Immunofluorescence - CBL Rabbit pAb (A0732)

Immunofluorescence analysis of NIH/3T3 cells using CBL Rabbit pAb (A0732) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CBL Rabbit pAb (A0732)}
Immunofluorescence - CBL Rabbit pAb (A0732)

Immunofluorescence analysis of PC-12 cells using CBL Rabbit pAb (A0732) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CBL Rabbit pAb (A0732)}
Immunoprecipitation - CBL Rabbit pAb (A0732)

Immunoprecipitation analysis of 600 μg extracts of Mouse thymus cells using 3 μg CBL antibody (A0732). Western blot was performed from the immunoprecipitate using CBL antibody at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on CBL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CBL. (Distance between topics and target gene indicate popularity.) CBL

* Data provided by citexs.com, for reference only.

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* For research use only. Not for therapeutic or diagnostic purposes.

Discontinued

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