Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human
Product name | Phospho-CBL-Y774 Antibody kit |
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Catalog No. | RK05788 |
Product name | Catalog No. | Positive Applications | Species Reactivity |
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Phospho-CBL-Y774 Rabbit pAb | AP0794 | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition | Human |
CBL Rabbit pAb | A0732 | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition | Human, Mouse, Rat |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 850-906 of human CBL (NP_005179.2). A synthetic phosphorylated peptide around Y774 of human CBL (NP_005179.2). |
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Sequence | AATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DGYDV |
Gene ID | 867 |
Swiss prot | P22681 |
Synonyms | CBL2; NSLL; C-CBL; RNF55; FRA11B; Phospho-CBL-Y774 |
Calculated MW | 100kDa |
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Observed MW | 110kDa |
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
Cellular location | Cell membrane,Cytoplasm |
Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (A0732).Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.
Western blot analysis of lysates from 293T cells, using CBL Rabbit pAb (A0732) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of lysates from Mouse thymus, using CBL Rabbit pAb (A0732) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
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Based on existing publications, the following genes are closely related to research on CBL. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to CBL. (Distance between topics and target gene indicate popularity.) CBL
* Data provided by citexs.com, for reference only.
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* For research use only. Not for therapeutic or diagnostic purposes.
Discontinued