Products > Catalog Antibodies > Polyclonal Antibodies

Parkin Rabbit pAb (A0968)

Publications (14) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Parkin Rabbit pAb (A0968)

Western blot analysis of extracts of various cell lines, using Parkin antibody (A0968) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Parkin Rabbit pAb (A0968)

Western blot analysis of extracts of 293T cells, using Parkin antibody (A0968) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Rat lung using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Human stomach using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Mouse kidney using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of Neuro-2a cells using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameParkin Rabbit pAb
Catalog No.A0968
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human Parkin (NP_004553.2).
SequenceNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHD
Gene ID5071
Swiss protO60260
SynonymsPRKN; AR-JP; LPRS2; PARK2; PDJ; Parkin
Calculated MW23kDa/30-51kDa
Observed MW52KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, PC-12, Mouse liver
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, cytosol
Customer validation

WB(Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

ABclonal:Western blot - Parkin Rabbit pAb (A0968)}

Western blot - Parkin Rabbit pAb (A0968)

Western blot analysis of extracts of various cell lines, using Parkin antibody (A0968) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Parkin Rabbit pAb (A0968)}

Western blot - Parkin Rabbit pAb (A0968)

Western blot analysis of extracts of 293T cells, using Parkin antibody (A0968) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)}

Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Rat lung using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)}

Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Human stomach using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Parkin Rabbit pAb (A0968)}

Immunohistochemistry - Parkin Rabbit pAb (A0968)

Immunohistochemistry of paraffin-embedded Mouse kidney using Parkin antibody (A0968) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Parkin Rabbit pAb (A0968)}

Immunofluorescence - Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of Neuro-2a cells using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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