Products > Catalog Antibodies > Polyclonal Antibodies

Parkin Polyclonal Antibody (A0968)

Datasheet

ABclonal:Western blot - Parkin Polyclonal Antibody (A0968)
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968)
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968)
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968)
ABclonal:Immunofluorescence - Parkin Polyclonal Antibody (A0968)

Overview

Product nameParkin Polyclonal Antibody
Catalog No.A0968
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Parkin
SequenceMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVGTGDTVVLRG
Gene ID5071
Swiss protO60260
SynonymsPRKN; AR-JP; LPRS2; PARK2; PDJ; E3 ubiquitin-protein ligase parkin
Calculated MW23kDa/30-51kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-12, Mouse brain
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, cytosol
ABclonal:Western blot - Parkin Polyclonal Antibody (A0968) }

Western blot - Parkin Polyclonal Antibody (A0968)

Western blot analysis of extracts of various cell lines, using Parkin antibody (A0968) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968) }

Immunohistochemistry - Parkin Polyclonal Antibody (A0968)

Immunohistochemistry of paraffin-embedded Rat lung using Parkin antibody (A0968) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968) }

Immunohistochemistry - Parkin Polyclonal Antibody (A0968)

Immunohistochemistry of paraffin-embedded Human stomach using Parkin antibody (A0968) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Parkin Polyclonal Antibody (A0968) }

Immunohistochemistry - Parkin Polyclonal Antibody (A0968)

Immunohistochemistry of paraffin-embedded Mouse kidney using Parkin antibody (A0968) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Parkin Polyclonal Antibody (A0968) }

Immunofluorescence - Parkin Polyclonal Antibody (A0968)

Immunofluorescence analysis of HeLa cells using Parkin antibody (A0968).

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