Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Pan Acetyl-Lysine Rabbit pAb (A2391)

Publications (31) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Immunofluorescence - Pan Acetyl-Lysine Rabbit pAb (A2391)

Immunofluorescence analysis of HeLa TSA and HeLa cells using Pan Acetyl-Lysine Rabbit pAb (A2391) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name Pan Acetyl-Lysine Rabbit pAb
Catalog No. A2391
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) . Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis . Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport . The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development .

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence containing acetylated K.
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW Refer to figures

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6
Cellular location
Customer validation

WB (Mus musculus, Homo sapiens)

Co-IP (Mus musculus, Homo sapiens)

IP (Danio rerio, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Pan Acetyl-Lysine Rabbit pAb images

ABclonal:Immunofluorescence - Pan Acetyl-Lysine Rabbit pAb (A2391)}

Immunofluorescence - Pan Acetyl-Lysine Rabbit pAb (A2391)

Immunofluorescence analysis of HeLa TSA and HeLa cells using Pan Acetyl-Lysine Rabbit pAb (A2391) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A2391 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on pan Acetyl-K. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to pan Acetyl-K. (Distance between topics and target gene indicate popularity.) pan Acetyl-K

* Data provided by citexs.com, for reference only.

Publishing research using A2391? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Alternative Products
Contact us to order