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PTPN14 Rabbit pAb (A12093)

Datasheet

ABclonal:Western blot - PTPN14 Polyclonal Antibody (A12093)

Overview

Product namePTPN14 Rabbit pAb
Catalog No.A12093
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 650-810 of human PTPN14 (NP_005392.2).
SequenceVRGMEAMTLKSLHLPMARRNTLREQGPPEEGSGSHEVPQLPQYHHKKTFSDATMLIHSSESEEEEEEAPESVPQIPMLREKMEYSAQLQAALARIPNKPPPEYPGPRKSVSNGALRQDQASLPPAMARARVLRHGPAKAISMSRTDPPAVNGASLGPSISE
Gene ID5784
Swiss protQ15678
SynonymsPTPN14; PEZ; PTP36
Calculated MW135kDa
Observed MW135kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, MCF7, A-549, K-562
Cellular locationCytoplasm, Nucleus, cytoskeleton
ABclonal:Western blot - PTPN14 Polyclonal Antibody (A12093) }

Western blot - PTPN14 Polyclonal Antibody (A12093)

Western blot analysis of extracts of various cell lines, using PTPN14 antibody (A12093) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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