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PSCA Polyclonal Antibody (A5614)

Datasheet

ABclonal:Western blot - PSCA Polyclonal Antibody (A5614)

Overview

Product namePSCA Polyclonal Antibody
Catalog No.A5614
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 12-114 of human PSCA (NP_005663.2).
SequenceLLCYSCKAQVSNEDCLQVENCTQLGEQCWTARIRAVGLLTVISKGCSLNCVDDSQDYYVGKKNITCCDTDLCNASGAHALQPAAAILALLPALGLLLWGPGQL
Gene ID8000
Swiss protO43653
SynonymsPSCA; PRO232; prostate stem cell antigen
Calculated MW12kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse kidney, Mouse heart, Mouse testis, Mouse eye
Cellular locationCell membrane, GPI-anchor, Lipid-anchor
ABclonal:Western blot - PSCA Polyclonal Antibody (A5614) }

Western blot - PSCA Polyclonal Antibody (A5614)

Western blot analysis of extracts of various cell lines, using PSCA antibody (A5614) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).

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