Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

AMPKα2 Mouse mAb (A0792)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - AMPKα2 Mouse mAb (A0792)

Western blot analysis of various lysates using AMPKα2 Mouse mAb (A0792) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name AMPKα2 Mouse mAb
Catalog No. A0792
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Immunogen information

Immunogen A synthetic peptide of human PRKAA2
Sequence Email for sequence
Gene ID 5563
Swiss prot P54646
Synonyms AMPK; AMPK2; PRKAA; AMPKa2; AMPKα2
Calculated MW 62kDa
Observed MW 68kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HeLa, HL-60, 293T
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Research Area

AMPKα2 Mouse mAb images

ABclonal:Western blot - AMPKα2 Mouse mAb (A0792)}

Western blot - AMPKα2 Mouse mAb (A0792)

Western blot analysis of various lysates using AMPKα2 Mouse mAb (A0792) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKAA2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAA2. (Distance between topics and target gene indicate popularity.) PRKAA2

* Data provided by citexs.com, for reference only.

Publishing research using A0792? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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