Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PPP1R15A Rabbit pAb (A16260)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP1R15A Rabbit pAb (A16260)

Western blot analysis of various lysates using PPP1R15A Rabbit pAb (A16260) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PPP1R15A Rabbit pAb (A16260)

Immunofluorescence analysis of H9C2 cells using PPP1R15A Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPP1R15A Rabbit pAb (A16260)

Immunofluorescence analysis of L929 cells using PPP1R15A Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PPP1R15A Rabbit pAb
Catalog No. A16260
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human PPP1R15A (NP_055145.3).
Sequence ASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG
Gene ID 23645
Swiss prot O75807
Synonyms GADD34; PPP1R15A
Calculated MW 73kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples U-87MG, U-937, HeLa, HepG2, Jurkat, mouse spleen, mouse lung, mouse thymus
Cellular location cytoplasm, cytosol, endoplasmic reticulum, Golgi apparatus, mitochondrial outer membrane, mitochondrion, protein phosphatase type 1 complex
Customer validation

WB (Danio rerio)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PPP1R15A Rabbit pAb images

ABclonal:Western blot - PPP1R15A Rabbit pAb (A16260)}

Western blot - PPP1R15A Rabbit pAb (A16260)

Western blot analysis of various lysates using PPP1R15A Rabbit pAb (A16260) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PPP1R15A Rabbit pAb (A16260)}

Immunofluorescence - PPP1R15A Rabbit pAb (A16260)

Immunofluorescence analysis of H9C2 cells using PPP1R15A Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPP1R15A Rabbit pAb (A16260)}

Immunofluorescence - PPP1R15A Rabbit pAb (A16260)

Immunofluorescence analysis of L929 cells using PPP1R15A Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PPP1R15A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPP1R15A. (Distance between topics and target gene indicate popularity.) PPP1R15A

* Data provided by citexs.com, for reference only.

Publishing research using A16260? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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