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PPP1CA Polyclonal Antibody (A2184)

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ABclonal:Western blot - PPP1CA Polyclonal Antibody (A2184)
ABclonal:Immunofluorescence - PPP1CA Polyclonal Antibody (A2184)
Review
ABclonal: review for PPP1CA Polyclonal Antibody(A2184)

Overview

Product namePPP1CA Polyclonal Antibody
Catalog No.A2184
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PPP1CA (NP_002699.1).
SequenceMSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK
Gene ID5499
Swiss protP62136
SynonymsPPP1CA; PP-1A; PP1A; PP1alpha; PPP1A; protein phosphatase 1 catalytic subunit alpha
Calculated MW32kDa/37kDa/38kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesDU145, MCF-7, A-549, NIH/3T3, HepG2, Mouse brain, Mouse kidney
Cellular locationCytoplasm, Nucleus, nucleolus, nucleoplasm
Customer validation

WB(Arabidopsis)

ABclonal:Western blot - PPP1CA Polyclonal Antibody (A2184) }

Western blot - PPP1CA Polyclonal Antibody (A2184)

Western blot analysis of extracts of various cell lines, using PPP1CA antibody (A2184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - PPP1CA Polyclonal Antibody (A2184) }

Immunofluorescence - PPP1CA Polyclonal Antibody (A2184)

Immunofluorescence analysis of U2OS cells using PPP1CA antibody (A2184). Blue: DAPI for nuclear staining.

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