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PPARγ Rabbit pAb (A0270)

Reviews (2)Publications (13) Datasheet

ABclonal:PPARγ Rabbit pAb
ABclonal:Western blot - PPARγ Rabbit pAb (A0270)

Western blot analysis of extracts of various cell lines, using PPARγ antibody (A0270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - PPARγ Rabbit pAb (A0270)

Western blot analysis of extracts of Rat fat, using PPARγ antibody (A0270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Rat heart using PPAR antibody (A0270) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Human colon using PPAR antibody (A0270) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Mouse lung using PPAR antibody (A0270) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of NIH/3T3 cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of PC-12 cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of U2OS cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for PPARγ Rabbit pAb(A0270)
Review
ABclonal: review for PPARγ Rabbit pAb(A0270)

Overview

Product namePPARγ Rabbit pAb
Catalog No.A0270
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPARγ (NP_056953.2).
SequenceLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFH
Gene ID5468
Swiss protP37231
SynonymsCIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; PPAR gamma; PPARG
Calculated MW21kDa/54kDa/57kDa
Observed MW53KDa/57KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-3, Mouse liver, Rat fat
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse Embryonic Stem Cells, Human liver cell)

WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Mesocricetus auratus)

IF(Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - PPARγ Rabbit pAb (A0270) }

Western blot - PPARγ Rabbit pAb (A0270)

Western blot analysis of extracts of various cell lines, using PPARγ antibody (A0270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - PPARγ Rabbit pAb (A0270) }

Western blot - PPARγ Rabbit pAb (A0270)

Western blot analysis of extracts of Rat fat, using PPARγ antibody (A0270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270) }

Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Rat heart using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270) }

Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Human colon using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PPARγ Rabbit pAb (A0270) }

Immunohistochemistry - PPARγ Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Mouse lung using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270) }

Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of NIH/3T3 cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270) }

Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of PC-12 cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPARγ Rabbit pAb (A0270) }

Immunofluorescence - PPARγ Rabbit pAb (A0270)

Immunofluorescence analysis of U2OS cells using PPARγ Rabbit pAb (A0270) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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